Journal
BIOSENSORS & BIOELECTRONICS
Volume 101, Issue -, Pages 213-218Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.10.032
Keywords
VEGF(165); Photoelectrochemical; DNA networks; Signal-off
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Funding
- National Natural Science Foundation of China [21501081, 21575116, 21473136]
- Fundamental Research Funds for the Central Universities [XDJK2017C021]
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We developed a novel signal-off photoelectrochemical (PEC) aptasensor based on an aptamer bridged DNA network structure for the sensitive detection of vascular endothelial growth factor (VEGF(165)), using g-C3N4 as photoactive material. The DNA network provides an excellent platform for the immobilization of methylene blue (MB), which can facilitate the electron transport through the DNA helix structure and suppress the recombination of electron-hole pairs generated by g-C3N4. In the presence of the target VEGF(165), the DNA network can be destroyed adequately by the recognition between VEGF(165) and the aptamer, resulting in the release of MB. Therefore, the originally enhanced electron transfer process could be inhibited, leading to a remarkable decrease of the photocurrent. A good linear relationship between the PEC signal and the logarithm of vEGF(165) concentration over the range of 100 fM to 10 nM with a detection limit of 30 fM can be obtained. Our concept can be easily extended to develop aptasensors for the sensitive detection of different targets by triggering the release of the payloads from their corresponding aptamer bridged DNA networks.
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