Journal
BIOSENSORS & BIOELECTRONICS
Volume 109, Issue -, Pages 139-149Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.03.010
Keywords
Electrochemical immunosensing; Graphene oxide-gold composite; Pathogen detection; Cronobacter sakazakii; Powdered infant formula
Categories
Funding
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT and Future Planning of Korea [2017M2A2A6A01020938, 2017R1D1A1B03035373, 2018R1A2B2006094]
- National Research Foundation of Korea [2017R1D1A1B03035373, 2017M2A2A6A01020938, 2018R1A2B2006094] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A sensitive electrochemical immunosensing platform for the detection of Cronobacter sakazakii was developed using a graphene oxide/gold (GO/Au) composite. Transmission electron microscopy showed that the Au nanoparticles, with an average size of < 30 nm, were well dispersed on the GO surface. For the detection of C. sakazakii, a polyclonal sakasakii antibody (IgG) was covalently immobilized to the Au nanoparticles on the surface of the GO/Au composite coated glassy carbon electrode (GCE). The electrochemical sensing performance of immunofunctionalized GCE was characterized by cyclic voltammetry and differential pulse voltammetry. Under optimized conditions, in pure culture there was a linear relationship between electrical signal and C. sakazakii levels over the range 2.0 x 10(2)-2.0 x 10(7) cfu/mL (R-2 = 0.999), with a detection limit of 2.0 x 10(1) cfu/mL. The total analytical time was 15 min per sample. The C. sakazakii electrochemical linmunosensing assay was able to successfully detect 2.0 x 10(1) cfu/mL of C. sakazakii in artificially contaminated powdered infant formula without any enrichment or pre-enrichment steps. Furthermore, the recovery rates of the C. sakazakii electrochemical immunosensing assay following spiking of powdered infant formula with different concentrations of C. sakazakii (cfu/mL) were 82.58% at 2.0 x 10(1) cfu/mL, 84.86% at 2.0 x 10(2) cfu/mL, and 95.40% at 2.0 x 10(3) cfu/mL. The C. sakazakii electrochemical immunosensing assay had good selectivity, reproducibility, and reactivity compared with other Cronobacter spp. and/or pathogens belonging to other genera, indicating its significant potential in the clinical diagnosis of C. sakazakii.
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