Journal
BIOSENSORS & BIOELECTRONICS
Volume 109, Issue -, Pages 123-131Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.02.047
Keywords
Functionalized carbon nanotubes; Ascorbic acid; Uric acid; Portable electrochemical sensor; Human fluids
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Funding
- Generalitat Valenciana [Prometeo2013/038]
- Ministerio de Economia y Competitividad [MAT2016-76595-R]
- Generalitat Valenciana
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A new portable electrochemical sensor based on 4-aminobenzoic acid-modified herringbone carbon nanotubes (hCNTs-4ABA/Au-IDA) has been developed for the simultaneous determination of ascorbic acid (AA) and uric acid (UA) in physiological fluids. AA and UA were quantified by chronoamperometry at 0.1 and 0.32 V, respectively, in phosphate buffer solution (PBS 0.25 M, pH 7.0). Significant results were obtained for the separate quantification of AA and UA, with a limit of detection (LOD) of 0.65 mu M for both analytes, and sensitivities of (9.0 +/- 0.4) A g(-1) mM(-1) and (8.8 +/- 0.3)A g(-1) mM(-1) for AA and UA, respectively. Repeatability was studied at 50 pM for AA and UA, providing relative standard deviations (RSD) lower than 9%. Additions of glucose, dopamine and epinephrine did not interfere with the AA and UA determination. Furthermore, UA did not interfere with AA determination at 0.1 V, although AA additions increased the current recorded at 0.32 V. The method has been successfully applied to human urine, perspiration and serum samples, without significant matrix effects, which allows for the use of an external calibration and the analysis of all the matrices investigated.
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