Journal
BIOSENSORS & BIOELECTRONICS
Volume 118, Issue -, Pages 108-114Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.07.038
Keywords
Graphene; SERS; Colon; Cancer; Label-Free; p53
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Funding
- UC-Riverside
- Alexander von Humboldt Foundation
- Zhejiang University Cao Guangbiao High-Tech Talent Fund
- Changjiang Chair Professorship of Xi'an Jiaotong University (China)
- United States Government [200008303]
- American people through the United States Department of State
- United States Agency for International Development (USAID) under the Pakistan - U.S. Science & Technology Cooperation Program
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Surface-Enhanced Raman Scattering (SERS) is used to differentiate two colon cancer cell line HCT 116, that is, to distinguish a TP53 gene knockout cell line (p53 -/-) from a wild type (p53 +/+). A label-free graphene/gold nanopyramid based SERS platform, combined with the multivariate analysis: principal component analysis, is used to profile live, dead, and burst colon cancer cells suspended in simulated body fluid (SBF). The graphene sheet permits SERS hotspot identification and provides a chemical enhancement for the biological constituents. This study found that a unique fingerprint exists for three different states of the cell, burst, live, and dead, which were used to differentiate the p53 +/+ and p53 -/- cell lines. Perceptron with Pocket Algorithm was also coupled with PCA to demonstrate an average of 81% sensitivity and 97% specificity in separating the two cell lines. The demonstration of single gene differentiation shows the great applicable potential of this SERS graphene hybrid platform for cancer diagnosis.
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