Journal
BIOSENSORS & BIOELECTRONICS
Volume 103, Issue -, Pages 138-142Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.12.033
Keywords
Enzyme-linked immunosorbent assay (ELISA); Optical sensor; Progesterone; Mobile ELISA (MELISA)
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Funding
- NSF Grant [1701081]
- USF Presidential Fellowship
- Div Of Industrial Innovation & Partnersh
- Directorate For Engineering [1701081] Funding Source: National Science Foundation
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Enzyme-linked immunosorbent assay (ELISA) is one of the most important technologies for biochemical analysis critical for diagnosis and monitoring of many diseases. Traditional systems for ELISA incubation and reading are expensive and bulky, thus cannot be used at point-of-care or in the field. Here, we propose and demonstrate a new miniature mobile phone based system for ELISA (MELISA). This system can be used to complete all steps of the assay, including incubation and reading. It weighs just 1 pound, can be fabricated at low cost, portable, and can transfer test results via mobile phone. We successfully demonstrated how MELISA can be calibrated for accurate measurements of progesterone and demonstrated successful measurements with the calibrated system.
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