Journal
BIOSENSORS & BIOELECTRONICS
Volume 100, Issue -, Pages 1-7Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.07.077
Keywords
Signal amplification; Layer-by-layer assemble; CdS QDs functionalized polystyrene; microspheres bioprobe; GO-PANI composite; Tumor cells detection; Electrochemical biosensor
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Funding
- Natural Science Foundation of Hebei Province [H2015203231]
- Youth Foundation
- Hebei Education Department of China [QN2014004]
- Key Research and Development Program of Qin Huangdao [201602A110]
- Yanshan University [B821]
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In this work, a novel ultrasensitive electrochemical biosensor was developed for the detection of K562 cell by a signal amplification strategy based on multiple layer CdS QDs functionalized polystyrene microspheres(PS) as bioprobe and graphene oxide(GO) -polyaniline(PANI) composite as modified materials of capture electrode. Due to electrostatic force of different charge, CdS QDs were decorated on the surface of PS by PDDA (poly(diallyldimethyl-ammonium chloride)) through a layer-by-layer(LBL) assemble technology, in which the structure of multiple layer CdS QDs increased the detection signal intensity. Moreover, GO-PANT composite not only enhanced the electron transfer rate, but also increased tumor cells load ratio. The resulting electrochemical biosensor was used to detect K562 cells with a lower detection limit of 3 cells mL(-1) (S/N = 3) and a wider linear range from 10 to 1.0 x 10(7) cells mL(-1). This sensor was also used for mannosyl groups on HeLa cells and Hct116 cells, which showed high specificity and sensitivity. This signal amplification strategy would provide a novel approach for detection, diagnosis and treatment for tumor cells.
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