Journal
BIOSENSORS & BIOELECTRONICS
Volume 105, Issue -, Pages 218-225Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.01.025
Keywords
Cerium dioxide; Electrochemical biosensor; Microfluidic paper-based analytical device; MicroRNA
Categories
Funding
- National Natural Science Foundation of China [21575051, 21775055, 21501090, 21475052]
- Natural Science Foundation of Shandong Province, China [2017C01-3]
- program for Taishan Scholars of Shandong province [ts201712048]
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In this work, an electrochemical biosensor based on Au nanorods (NRs) modified microfluidic paper-based analytical devices (mu PADs) were constructed for sensitive detection of microRNA (miRNA) by using cerium dioxide - Au@glucose oxidase (CeO2-Au@GOx) as an electrochemical probe for signal amplification. Au NRs were synthesized by in-situ growth method in mu PADs surface to enhance the conductivity and modified hairpin probe through Au-S bonds. The construction of the signal transducer layer was carried out by GOx catalyzing glucose to produce H2O2, which was further electrocatalyzed by CeO2. After the biosensor was constructed, an obvious electrochemical signal was observed from the reduction of H2O2. In order to make the detection more convincing, the visual detection was performed based on the oxidation of 3,3',5,5'-tetramethylbenzidine by H2O2 with the help of Exonuclease I. The electrochemical biosensor provided a wide linear range of 1.0 fM to 1000 fM with a relatively low detection limit of 0.434 fM by the electrochemical measurement. Linear range of 10 fM to 1000 fM with a relatively low detection limit of 7.382 fM was obtained by visual detection. The results indicated the proposed platform has potential utility for detection of miRNA.
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