Journal
BIOSENSORS & BIOELECTRONICS
Volume 101, Issue -, Pages 7-13Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.09.043
Keywords
Dual-type responsive electrochemical immunosensor; PCSK9; Pt-poly (methylene blue) nanocomposites; N-C-60-PdPt; N-GNRs
Categories
Funding
- National Nature Science Foundation of China [81370403]
- Chongqing foundation and advanced research project [CSTC2015jcyjBX0053]
- Chongqing precision medical key technology research and development and demonstration projects [cstc2016shms-ztzx0042]
- Chongqing medical university scientific research cultivating fund [201414]
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In this study, a dual-type responsive electrochemical immunosensor was developed for the quantitative detection of proprotein convertase subtilisin/kexin type 9 (PCSK9), a potential biomarker of cardiovascular disease in serum. N-doped graphene nanoribbons (N-GNRs) with good conductivity were used as the sensing matrix modifying the glassy carbon electrode. Palladium platinum alloy (PdPt) nanoparticles with high catalytic performance toward the reduction of hydrogen peroxide (H2O2) were reduced onto amino-functionalized fullerene (n-C-60-PdPt) and significantly amplified the electrochemical signal recorded by the amperometric i-t curve. Furthermore, staphylococcus protein A (SPA) with antibody orientation function was introduced to improve the immunoreaction efficiency. Accordingly, a label-free immunosensor was fabricated based on n-C-60-PdPt/NGNRs for the quick detection of PCSK9. Meanwhile, to realize ultrasensitive detection of PCSK9, Pt-poly (methylene blue) (Pt-PMB) nanocomposites synthesized by a one-pot method for the first time were used as a novel signal label, which exhibited uniform morphology as well as good conductivity and produced an electrochemical signal recorded by differential pulse voltammetry (DPV). Herein, a novel sandwich-type immunosensor was designed using n-C-60-PdPt/N-GNRs as the sensing matrix and Pt-PMB as the signal label for sensitive detection of PCSK9. Under optimal conditions, the label-free immunosensor showed a linear range of 10 pg mL(-1) to 100 ng mL(-1) with a detection limit of 3.33 pg mL(-1) (S/N = 3), and the sandwich-type immunosensor exhibited a linear range of 100 fg mL(-1) to 100 ng mL(-1) with a detection limit of 0.033 pg mL(-1) (S/N = 3) for PCSK9 detection, indicating its potential application in clinical bioassay analysis.
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