Journal
BIOSENSORS & BIOELECTRONICS
Volume 101, Issue -, Pages 199-205Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.10.030
Keywords
Endotoxin; Lipopolysaccharide; Septic shock; Au atomic cluster; Aptasensor
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Funding
- Kerala State Council for Science, Environment, and Technology (KSCSTE) [003/SRSPS/2014/KSCSTE]
- Department of Science and Technology (DST) [SR/FTP/CS-28/2006]
- CSIR [01(2762)/13/EMR-II]
- University Grant Commission
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We have constructed an aptamer immobilized gold atomic cluster mediated, ultrasensitive electrochemical biosensor (Apt/MAC/Au) for LPS detection without any additional signal amplification strategy. The aptamer self-assemble onto the gold atomic clusters makes Apt/AuAC/Au an excellent platform for the LPS detection. Differential pulse voltammetry and EIS were used for the quantitative LPS detection. The Apt/AuAC/Au sensor offers an ultrasensitive and selective detection of LPS down to 7.94 x 10(-21) M level with a wide dynamic range from 0.01 attomolar to 1 pM. The sensor exhibited excellent selectivity and stability. The real sample analysis was performed by spiking the diluted insulin sample with various concentration of LPS and obtained recovery within 2% error value. The sensor is found to be more sensitive than most of the literature reports. The simple and easy way of construction of this sensor provides an efficient and promising detection of an even trace amount of LPS.
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