Journal
BIOSENSORS & BIOELECTRONICS
Volume 109, Issue -, Pages 214-223Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.03.012
Keywords
Wireless actuation; Centrifugo-pneumatic valves; Lab-on-a-Disc; Centrifugal platform; Automation; Closed-loop control
Categories
Funding
- National Council of Science and Technology, CONACyT (Mexico)
- University of Freiburg (Germany)
- Karlsruhe Institute of Technology (Germany)
- Sao Paulo Research Foundation (FAPESP, Brazil) [2015/16311-0]
- European Union [FP7-KBBE-2013-7-613908-DECATHLON, H2020-FETOPEN-1-2016-2017-737043-TISuMR]
- Science Foundation Ireland (SFI)
- Fraunhofer-Gesellschaft under the SFI Strategic Partnership Programme [16/SPP/3321]
- Science Foundation Ireland (SFI) [16/SPP/3321] Funding Source: Science Foundation Ireland (SFI)
Ask authors/readers for more resources
In this paper we present a wirelessly powered array of 128 centrifugo-pneumatic valves that can be thermally actuated on demand during spinning. The valves can either be triggered by a predefined protocol, wireless signal transmission via Bluetooth, or in response to a sensor monitoring a parameter like the temperature, or homogeneity of the dispersion. Upon activation of a resistive heater, a low-melting membrane (Parafilm (TM)) is removed to vent an entrapped gas pocket, thus letting the incoming liquid wet an intermediate dissolvable film and thereby open the valve. The proposed system allows up to 12 heaters to be activated in parallel, with a response time below 35, potentially resulting in 128 actuated valves in under 30 s. We demonstrate, with three examples of common and standard procedures, how the proposed technology could become a powerful tool for implementing diagnostic assays on Lab-on-a-Disc. First, we implement wireless actuation of 64 valves during rotation in a freely programmable sequence, or upon user input in real time. Then, we show a closed-loop centrifugal flow control sequence for which the state of mixing of reagents, evaluated from stroboscopically recorded images, triggers the opening of the valves. In our last experiment, valving and closed-loop control are used to facilitate centrifugal processing of whole blood.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available