Journal
BIOSENSORS & BIOELECTRONICS
Volume 99, Issue -, Pages 136-141Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.07.049
Keywords
Molecularly imprinted polymers; Electrochemical biosensor; Bovine serum albumin; Thermo-responsive memory hydrogel
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Funding
- National Natural Science Foundation of China [21475046, 21427809, 21645004]
- Fundamental Research Funds for the Central Universities [2015ZP028, 2017MS094]
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Herein, the self-cleaned electrochemical protein imprinting biosensor basing on a thermo-responsive memory hydrogel was constructed on a glassy carbon electrode (GCE) with a free radical polymerization method. Combining the advantages of thermo-responsive molecular imprinted polymers and electrochemistry, the resulted biosensor presents a novel self-cleaned ability for bovine serum albumin (BSA) in aqueous media. As a temperature controlled gate, the hydrogel film undergoes the adsorption and desorption of BSA basing on a reversible structure change with the external temperature stimuli. In particular, these processes have been revealed by the response of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) of electroactive [Fe(CN)(6)](3-/4-). The results have been supported by the evidences of scanning electron microscopy (SEM) and contact angles measurements. Under the optimal conditions, a wide detection range from 0.02 mu mol L-1 to 10 mu mol L-1 with a detection limit of 0.012 mu mol L-1 (S/N = 3) was obtained for BSA. This proposed BSA sensor also possesses high selectivity, excellent stability, acceptable recovery and good reproducibility in its practical applications.
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