Journal
BIOSENSORS & BIOELECTRONICS
Volume 101, Issue -, Pages 52-59Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.10.003
Keywords
Bacterial capture; Bacteria detection; Impedance sensor; Antibacterial nanoparticles; Prickly nanostructure
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Funding
- National Natural Science Foundation of China [21576120, U1607125, 21574091]
- Natural Science Foundation of Jiangsu Province [BK20160056, BK20131223, BK20150433]
- National Postdoctoral Science Foundation [2013M530240, 2014T70480]
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In this work, we demonstrated a nano-decorated porous impedance electrode sensor for efficient capture, rapid killing and ultrasensitive detection of bacteria. The multi-functional sensor was prepared by a facile sono-chemical method via in situ deposition of antibacterial prickly Zn-CuO nanoparticles and graphene oxide (GO) nanosheets on a Ni porous electrode. Due to the surface burr-like nanostructures, the nano-decorated impedance sensor exhibited very good bacterial-capture efficiency (70 - 80% in 20 min) even at a low concentration of 50 CFU mL(-1), rapid antibacterial rate (100% killing in 30 min) and high detection sensitivity (as low as 10 CFU mL(-1)). More importantly, the nano-decorated sensor has proven to be highly effective in quantitative detection of bacteria in a biological sample, for example, a rat blood sample spiked with E. coll. Despite the complexity of blood, the sensor still exhibited excellent detection precision within 30 min at bacteria concentrations ranging from 10 - 10(5) CFU mL(-1). The simplicity, rapidity, sensitivity, practicability and multifunctionality of this impedance sensor would greatly facilitate applications in portable medical devices for on-the-spot diagnosis and even the possibility for simultaneous therapy of diseases caused by bacterial infections.
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