Journal
BIOSENSORS & BIOELECTRONICS
Volume 104, Issue -, Pages 79-86Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.01.002
Keywords
3, 6-diamino-9-ethylcarbazole; Molecularly imprinted polymer; 17-beta-estradiol
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Funding
- National Natural Science Foundation of China [81503037]
- Shenyang Pharmaceutical University [ZQN2015029]
- Doctoral Research Funding of Liaoning Province [201601141]
- Basic research projects of Liaoning Provincial Department of Education [2017LQN06]
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In this work, we reported the synthesis of 3, 6-diamino-9-ethylcarbazole and its application as a new monomer for preparation of molecularly imprinted polymer (MIP) electrochemical sensor. The as prepared MIP sensor exhibited ultrahigh sensitivity and selectivity for the detection of 17-beta-estradiol in attomolar levels (1 x 10(-18) mol L-1). The sensor works by detecting the change of the interfacial impedance that is derived from recognition of 17-beta-estradiol on the MIP layer. The MIP sensor based on 3, 6-diamino-9-ethylcarbazole monomer revealed better performance than that of unmodified carbazole monomer. The monomer/template ratio, electropolymerization scanning cycles, and the incubation pH values were optimised in order to obtain the best detection efficiency. Under the optimised condition, the MIP sensor exhibits a wide linear range from 1 aM to 10 mu M (1 x 10(-18)-1 x 10(-5) mol L-1). A low detection limit of 0.36 aM (3.6 x 10(-19) mol L-1) and a good selectivity towards structurally similar compounds were obtained. The proposed MIP sensor also exhibits long-term stability and applicability in human serum samples. These advantages enabled this MIP sensor to be a promising alternative of electrochemical sensor and may be extended to detection of other endogenous compounds.
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