Journal
BIOSENSORS & BIOELECTRONICS
Volume 115, Issue -, Pages 83-90Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.05.019
Keywords
Nucleic acid tests; Malaria; Point-of-care; Blood; Centrifuge-free; Lab-on-a-disc
Categories
Funding
- National Institutes of Health, USA [U19AI089672]
- National Science Foundation, USA [ECCS-1710831]
- Penn State Award 'Materials Matter at the Human Level'
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U19AI089672] Funding Source: NIH RePORTER
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The effectiveness of malaria screening and treatment highly depends on the low-cost access to the highly sensitive and specific malaria test. We report a real-time fluorescence nucleic acid testing device for malaria field detection with automated and scalable sample preparation capability. The device consists a compact analyzer and a disposable microfluidic reagent compact disc. The parasite DNA sample preparation and subsequent realtime LAMP detection were seamlessly integrated on a single microfluidic compact disc, driven by energy efficient non-centrifuge based magnetic field interactions. Each disc contains four parallel testing units which could be configured either as four identical tests or as four species-specific tests. When configured as species-specific tests, it could identify two of the most life-threatening malaria species (P. falciparum and P. vivax). The NAT device is capable of processing four samples simultaneously within 50 min turnaround time. It achieves a detection limit of similar to 0.5 parasites/mu I for whole blood, sufficient for detecting asymptomatic parasite carriers. The combination of the sensitivity, specificity, cost, and scalable sample preparation suggests the real-time fluorescence TAMP device could be particularly useful for malaria screening in the field settings.
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