Journal
BIOSENSORS & BIOELECTRONICS
Volume 115, Issue -, Pages 77-82Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.05.006
Keywords
Electrochemiluminescence; Multiplexing immunoassay; Dual-wavebands-resolved; Photomultiplier-tube; Dichroic mirror
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Funding
- National Natural Science Foundation of China [21427808, 21375077]
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A dual-wavebands-resolved electrochemiluminescence (ECL) multiplexing immunoassay (MIA) was developed for simultaneously detecting alpha fetoprotein antigen (AFP) in greenish waveband with CdSe550 (lambda(max) = 550 nm) nanocrystals (NCs) and carbohydrate antigen 125 (CA125) in near-infrared waveband with CdTe790 (lambda(max) = 790 nm) NCs via one-pot ECL reaction, in which dichroic mirror works as a key part to reflect ECL from CdSe550 to one photomultiplier-tube (PMT) and transmit ECL from CdTe790 to the other PMT for dual-wavebands-resolved assay. The proposed ECL-MIA strategy was capable of simultaneously determining AFP with linearly response from 5 pg/mL to 5 ng/mL and limit of detection at 1 pg/mL, and CA125 with linearly response from 5 mU/mL to 1 U/mL and limit of detection at 1 mU/mL, with desired specificity and without obvious energy-transfer between ECL tags. The dichroic mirror assistant ECL setup is easy-to-assemble and convenient for the popularization of color-resolved multiplexing ECL assay.
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