Journal
BIOSENSORS & BIOELECTRONICS
Volume 111, Issue -, Pages 159-165Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.04.005
Keywords
Cellular microarray; Dielectrophoresis; Cell counting; Cell viability; Impedance spectroscopy
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Funding
- Asian Office of Aerospace Research and Development (AOARD, Japan) [FA2386-16-1-4072]
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Microarray technology to isolate living cells using external fields is a facile way to do phenotypic analysis at the cellular level. We have used alternating current dielectrophoresis (AC-DEP) to drive the assembly of live pathogenic Salmonella typhi (S.typhi) and Escherichia coli (E.coli) bacteria into miniaturized single cell micro arrays. The effects of voltage and frequency were optimized to identify the conditions for maximum cell capture which gave an entrapment efficiency of 90% in 60 min. The chip was used for calibration-free estimation of cellular loads in binary mixtures and further applied for rapid and enhanced testing of cell viability in the presence of drug via impedance spectroscopy. Our results using a model antimicrobial sushi peptide showed that the cell viability could be tested down to 5 mu g/mL drug concentration under an hour, thus establishing the utility of our system for ultrafast and sensitive detection.
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