Journal
BIOSENSORS & BIOELECTRONICS
Volume 113, Issue -, Pages 16-24Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.04.050
Keywords
NiOxFey@mC composite; Hollow NiFe PBA; Electrochemical aptasensor; Ultra-sensitivity; Adenosine triphosphate
Categories
Funding
- National Natural Science Foundation of China (NSFC) [U1604127, 21601161]
- Innovative Technology Team of Henan Province [CXTD2014042]
Ask authors/readers for more resources
We designed and constructed a novel aptasensor based on the porous nanostructured bimetallic NiFe-oxides embedded with the mesoporous carbon (represented by NiOxFeOy@mC) for sensitively detecting adenosine triphosphate (ATP), of which the porous NiOxFeOy@mC was derived from the hollow NiFe Prussian blue analogue (hollow NiFe PBA) by calcinating under high temperature. Owning to the excellent electrochemical activity originated from the metal oxides and mesoporous carbon and the strong binding interaction between the aptamer strands and the nanostructure hybrid, the formed porous NiOxFeOy@mC composite calcinated at 900 degrees C exhibited superior sensitivity toward ATP determination in comparison with other porous nanocubes obtained at 500 and 700 degrees C. The proposed aptasensor not only revealed a wide linear range from 5.0 fg.mL(-1) to 5.0 ng with a extremely low detection limit of 0.98 fg.mL(-1) (1.62 fM) (S/N = 3), but also displayed high selectivity towards other interferences, good stability and reproducibility, and acceptable applicability. Therefore, this proposed approach provides a promising platform for ultra-sensitive detection of ATP, further having the potential applications on diagnosis of ATP-related diseases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available