Journal
BIORESOURCE TECHNOLOGY
Volume 267, Issue -, Pages 743-755Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.07.092
Keywords
Biofuels; Oleaginous microbes; Biomass; Cell disruption; Lipid extraction
Funding
- Dave C. Swalm School of Chemical Engineering and Bagley College of Engineering, Mississippi State University
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Lipid extraction directly from the wet oleaginous microorganisms for biodiesel production is preferred as it reduces the energy input for traditional processes which require extensive drying of the biomass prior to the extraction. The high water content (>= 80% on cell dry weight) in the wet biomass hinders the extraction efficiency due to the mass transfer limitation. This limitation can be overcome by pretreating wet biomass prior to the lipid extraction using pressurized gas that can be used alone or combined with other pretreatments to disrupt the cell wall. In this review, an extensive discussion on different pretreatments and the subsequent lipid extraction using these pretreatments is presented. Furthermore, a detailed account of the cell disruption using pressurized gas (e.g., CO2) treatment for microbial cell lysing is also presented. Finally, a new technique on lipid extraction directly from wet biomass using the combination of pressurized CO2 and microwave pretreatment is proposed.
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