Journal
BIORESOURCE TECHNOLOGY
Volume 254, Issue -, Pages 278-283Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.01.036
Keywords
Electrochemical synthesis of formate; Microbial electrosynthesis; Enzyme electrode; Heterodisulfide reductase; Direct electron transfer
Funding
- Office of Naval Research (USA) [N000141612240]
- Emil Aaltonen Foundation (Finland)
- Academy of Finland (Finland) [277121]
- U.S. Department of Defense (DOD) [N000141612240] Funding Source: U.S. Department of Defense (DOD)
- Academy of Finland (AKA) [277121, 277121] Funding Source: Academy of Finland (AKA)
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Electrosynthesis of formate is a promising technology to convert CO2 and electricity from renewable sources into a biocompatible, soluble, non-flammable, and easily storable compound. In the model methanogen Methanococcus maripaludis, uptake of cathodic electrons was shown to proceed indirectly via formation of formate or H-2 by undefined, cell-derived enzymes. Here, we identified that the multi-enzyme heterodisulfide reductase supercomplex (Hdr-SC) of M. maripaludis is capable of direct electron uptake and catalyzes rapid H-2 and formate formation in electrochemical reactors (-800 mV vs Ag/AgCl) and in Fe(0) corrosion assays. In Fe(0) corrosion assays and electrochemical reactors, purified Hdr-SC primarily catalyzed CO2 reduction to formate with a coulombic efficiency of 90% in the electrochemical cells for 5 days. Thus, this report identified the first enzyme that stably catalyzes the mediator-free electrochemical reduction of CO2 to formate, which can serve as the basis of an enzyme electrode for sustained electrochemical production of formate.
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