Journal
BIORESOURCE TECHNOLOGY
Volume 250, Issue -, Pages 256-264Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.11.023
Keywords
Bioethanol; Cheese whey; Lignocellulose biomass; beta-Galactosidase; High solid loadings
Funding
- Portuguese Foundation for Science and Technology (FCT) [UID/BIO/04469/2013]
- COMPETE [POCI-01-0145-FEDER-006684]
- BioTecNorte operation - European Regional Development Fund under the scope of Norte - Programa Operacional Regional do Norte [NORTE-01-0145-FEDER-000004]
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The mixture of Eucalyptus globulus wood (EGW) and cheese whey powder (CWP) was proposed for intensification of simultaneous saccharification and fermentation (SSF) at high temperature and solid loadings using the industrial Saccharomyces cerevisiae Ethanol Red (R) strain. High ethanol concentration (93 g/L), corresponding to 94% ethanol yield, was obtained at 35 degrees C from 37% of solid mixture using cellulase and beta-galactosidase enzymes (24.2 FPU/g and 20.0 U/g, respectively). The use of CWP mixed with pretreated EGW increased the ethanol concentration in 1.5-fold, in comparison with SSF experiments without CWP for both Ethanol Red (R) and CEN.PK113-7D strains. Moreover, 1.4-fold higher ethanol concentration was obtained with Ethanol Red (R), in comparison with CEN.PK113-7D strain. Ethanol Red (R) strain was genetically engineered for beta-galactosidase production in order to advance towards a fully integrated process. This work shows the feasibility of attaining high ethanol concentrations in second generation bioprocesses by a multi-waste valorization approach.
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