Journal
BIORESOURCE TECHNOLOGY
Volume 250, Issue -, Pages 35-42Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.09.208
Keywords
Duckweed; Succinic acid; Actinobacillus succinogenes; Fermentation; Semi-simultaneous saccharification and fermentation (SSSF)
Funding
- Natural Science Foundation of China [31660022]
- Natural Science Foundation of Guangxi Province, China [2016GXNSFBA380205]
- Science and Technology Development Project of Nanning [20071004-1]
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A novel process of enzyme pretreatment and semi-simultaneous saccharification and fermentation (SSSF) was developed in this work to improve succinic acid (SA) productivity from duckweed (Landoltia punctata) and achieve low viscosity. Viscosity (83.86%) was reduced by the pretreatment with combined enzymes at 50 degrees C for 2 h to a greater extent than that by single enzyme (26.19-71.75%). SSSF was an optimal combination with 65.31 g/L of SA content, which was remarkably higher than those obtained through conventional separate hydrolysis and fermentation (62.12 g/L) and simultaneous saccharification and fermentation (52.41 g/L). The combined approach was effective for SA production. Approximately 75.46 g/L of SA content with a yield of 82.87% and a productivity of 1.35 g/L/h was obtained after 56 h in a 2 L bioreactor. Further studies will focus on increasing the working scale of the proposed method.
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