Journal
BIORESOURCE TECHNOLOGY
Volume 247, Issue -, Pages 1184-1188Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.08.166
Keywords
2,5-Furandicarboxylic acid; Biotransformation; Raoultella ornithinolytica BF60; 5-Hydroxymethylfurfural oxidase; HMF/furfural oxidoreductase
Funding
- National Natural Science Foundation of China [31622001, 31671845, 31600068]
- Natural Science Foundation of Jiangsu Province [BK20160176]
- 111 Project [111-2-06]
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2,5-Furandicarboxylic acid (FDCA) is a promising bio-based building block and can be produced by biotransformation of 5-hydroxymethylfurfural (HMF). To improve the FDCA production, two genes-one encoding HMF oxidase (HMFO; from Methylovorus sp. strain MP688) and another encoding for HMF/Furfural oxidoreductase (HmfH; from Cupriavidus basilensis HMF14)-were introduced into Raoultella ornithinolytica BF60. The FDCA production in the engineered whole-cell biocatalyst increased from 51.0 to 93.6 mM, and the molar conversion ratio of HMF to FDCA increased from 51.0 to 93.6%.
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