Journal
BIORESOURCE TECHNOLOGY
Volume 247, Issue -, Pages 616-623Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.09.148
Keywords
Methane; Ammonia inhibition; Microbial community; Syntrophic acetate oxidizer; Methanosarcina
Funding
- Energinet.dk under the project ForskEL MicrobStopNH3-Innovative bioaugmentation strategies to tackle ammonia inhibition in anaerobic digestion process [2015-12327]
- Spanish Ministry of Economy and Competitiveness [ENE2013-45416-R, RYC-2014-16823]
- China Scholarship Council
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Acclimatized anaerobic communities to high ammonia levels can offer a solution to the ammonia toxicity problem in biogas reactors. In the current study, a stepwise acclimation strategy up to 10 g NH4+-NL-1, was performed in mesophilic (37 +/- 1 degrees C) continuously stirred tank reactors. The reactors were co-digesting (20/80 based on volatile solid) cattle slurry and microalgae, a protein-rich, 3rd generation biomass. Throughout the acclimation period, methane production was stable with more than 95% of the uninhibited yield. Next generation 16S rRNA gene sequencing revealed a dramatic microbiome change throughout the ammonia acclimation process. Clostridium ultunense, a syntrophic acetate oxidizing bacteria, increased significantly alongside with hydrogenotrophic methanogen Methanoculleus spp., indicating strong hydrogenotrophic methanogenic activity at extreme ammonia levels (> 7 g NH4+-NL-1). Overall, this study demonstrated for the first time that acclimation of methanogenic communities to extreme ammonia levels in continuous AD process is possible, by developing a specialised acclimation AD microbiome.
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