Journal
BIORESOURCE TECHNOLOGY
Volume 247, Issue -, Pages 88-95Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.08.192
Keywords
Inonotus obliquus; Cellulolytic enzymes; Ligninolytic enzyme; Solid state fermentation; Optimal conditions; Saccharification
Funding
- Zhejiang Provincial Natural Science Foundation, China [LY16B020013]
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White rot fungi have been usually considered for lignin degradation and ligninolytic enzyme production. To understand whether the white rot fungus Inonotus obliquus was able to produce highly efficient cellulase system, the production of cellulolytic enzyme cocktails was optimized under solid state fermentation. The activities of CMCase, FPase, and beta-glucosidase reached their maximum of 27.15 IU/g, 3.16 IU/g and 2.53 IU/g using wheat bran at 40% (v/w) inoculum level, initial pH of 6.0 and substrate-moisture ratio of 1:2.5, respectively. The enzyme cocktail exhibited promising properties in terms of high catalytic activity at 40-60 degrees C and at pH 3.0-4.5, indicating that the cellulolytic enzymes represent thermophilic and acidophilic characteristics. Saccharification of raw wheat straw and rice straw by the cellulolytic enzyme cocktail sampled on Day 12 resulted in the release of reducing sugar of 130.24 mg/g and 125.36 mg/g of substrate after 48 h of hydrolysis, respectively.
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