Journal
BIORESOURCE TECHNOLOGY
Volume 265, Issue -, Pages 59-65Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.05.108
Keywords
Laccase pretreatment; Enzymatic hydrolysis; Detoxification; Sequential protocol; Agrofood waste valorization
Funding
- European Union's research and innovation program Horizon 2020 [654623]
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Apple pomace, potato peels, and coffee silverskin are attractive agrofood wastes for the production of biofuels and chemicals, due to their abundance and carbohydrate content. As lignocellulosic biomasses, their conversion is challenged by the presence of lignin that prevents hydrolysis of polysaccharides, hence demanding a pretreatment step. In this work, the effectiveness of Pleurotus ostreatus laccases (with and without mediator) to remove lignin, improving the subsequent saccharification, was assessed. Optimized conditions for sequential protocol were set up for all agrofood wastes reaching delignification and detoxification yields correlated with high saccharification. Especially noteworthy were results for apple pomace and coffee silverskin for which 83% of and 73% saccharification yields were observed, by using laccase and laccase mediator system, respectively. The herein developed sequential protocol, saving soluble sugars and reducing the amount of wastewater, can improve the overall process for obtaining chemicals or fuels from agrofood wastes.
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