Journal
BIORESOURCE TECHNOLOGY
Volume 257, Issue -, Pages 92-101Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.02.056
Keywords
Ralstonia eutropha; Polyhydroxyalkonate; Co-culture; Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); Consortia
Funding
- KU Research Professor Program of Konkuk University, Seoul, South Korea
- National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2015M1A5A1037196, 2017R1D1A1B03030766]
- National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2017M3A9E4077234]
- Korea Institute of Energy Technology Evaluation and Planning (KETEP)
- Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea [20163010092150]
- Korea Evaluation Institute of Industrial Technology (KEIT) [20163010092150] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2015M1A5A1037196, 2017R1D1A1B03030766, 31Z20150113465, 2017M3A9E4077234] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
Ralstonia eutropha is a well-known microbe reported for polyhydroxyalkonate (PHA) production, and unable to utilize sucrose as carbon source. Two strains, Ralstonia eutropha H16 and Ralstonia eutropha 5119 were co-cultured with sucrose hydrolyzing microbes (Bacillus subtilis and Bacillus amyloliquefaciens) for PHA production. Co-culture of B. subtilis: R. eutropha 5119 (BS:RE5) resulted in best PHA production (45% w/w dcw). Optimization of the PHA production process components through response surface resulted in sucrose: NH4Cl:B. subtilis:R. eutropha (3.0:0.17:0.10:0.190). Along with the hydrolysis of sucrose, B. subtilis also ferments sugars into organic acid (propionic acid), which acts as a precursor for HV monomer unit. Microbial consortia of BS:RE5 when cultured in optimized media led to the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV) with 66% w/w of dcw having 16 mol% HV fraction. This co-culture strategy overcomes the need for metabolic engineering of R. eutropha for sucrose utilization, and addition of precursor for copolymer production.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available