Journal
BIORESOURCE TECHNOLOGY
Volume 258, Issue -, Pages 12-17Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.02.124
Keywords
Cellulase; Inhibition; Enzymatic hydrolysis; Steam pretreatment; Carbocation scavengers
Funding
- Natural Science Foundation of Jiangsu Province, China [BK20170832]
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The aim of this work was to study how to minimize cellulase inhibition of whole slurry biomass hydrolysis through addition of carbocation scavengers during acid-catalyzed pretreatment. Various potential carbocation scavengers were compared and their inhibition mitigating effects towards the hydrolytic performance of cellulase enzymes was assessed. The results indicated that the addition of carbocation scavengers during the pretreatment process could not only alleviate the inhibitory effect of the phenolics on the enzymatic hydrolysis but also increase the accessibility of cellulases to the pretreated substrates. It appeared that lignin-derived compounds such as 4-hydroxybenzoic acid, vanillic acid, syringic acid could all serve as efficient scavengers to alleviate the inhibitory effect of phenolics on cellulose hydrolysis where the syringic acid showed the best mitigating effect. By combining the carbocation scavengers in the pretreatment process, an improved cellulose hydrolysis of the pretreated whole slurry could be achieved without any post detoxification step.
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