Journal
BIORESOURCE TECHNOLOGY
Volume 256, Issue -, Pages 548-551Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.02.074
Keywords
Haematococcus pluvialis; Astaxanthin; Algal biomass; Carbon dioxide; Stress induction
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In this study, an economical two-stage method was proposed for the production of natural astaxanthin from Haematococcus pluvialis without a medium replacement step. In stage 1, H. pluvialis were grown under low light illumination until they reached optimal biomass. In stage 2, cells were switched to astaxanthin induction conditions utilizing the combination of high light illumination and elevated carbon dioxide levels (5 or 15%). The introduction of CO2 altered the C/N balance creating a nutrient deficiency without a change of media. The resulting astaxanthin yield was 2-3 times that of using either stressor alone. This astaxanthin induction method has many advantages over current methods including no medium replacement and a short induction time of less than four days.
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