Journal
BIORESOURCE TECHNOLOGY
Volume 257, Issue -, Pages 54-61Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.02.028
Keywords
Penicillium chrysogenum; Secretome; Lignocellulosic biomass; Enzymatic hydrolysis; Synergistic effect
Funding
- Project of State Key Laboratory of Agrobiotechnology [2017SKLAB7-15]
- Shanxi 1331 Project of Collaborative Innovation Centre
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Herein, we report the secretome of Penicillium chrysogenum P33 under induction of lignocellulose for the first time. A total of 356 proteins were identified, including complete cellulases and numerous hemicellulases. Supplementing a commercial cellulase with increasing dosage of P33 enzyme cocktail from 1 to 5 mg/g substrate increased the release of reducing sugars from delignified corn stover by 21.4% to 106.8%. When 50% cellulase was replaced by P33 enzyme cocktail, release of reducing sugars was 78.6% higher than with cellulase alone. Meanwhile, glucan and xylan conversion was increased by 37% and 106%, respectively. P33 enzyme cocktail also enhanced commercial cellulase hydrolysis against four different delignified lignocellulosic biomass. These findings demonstrate that mixing appropriate amount of P33 cocktail with cellulase improves polysaccharide hydrolysis, suggesting P33 enzymes have great potential for industrial applications.
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