Journal
BIORESOURCE TECHNOLOGY
Volume 257, Issue -, Pages 121-128Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.01.153
Keywords
Direct transesterification; Lipids; Solid-phase extraction; Thin-layer chromatography; Wastewater
Funding
- Swedish Energy Agency [38239-1]
- EU Interreg Botnia-Atlantica
- Kempe Foundation
Ask authors/readers for more resources
A systematic qualitative and quantitative analysis of fatty acid methyl esters (FAMEs) is crucial for microalgae species selection for biodiesel production. The aim of this study is to identify the best method to assess microalgae FAMEs composition and content. A single-step method, was tested with and without purification steps-that is, separation of lipid classes by thin-layer chromatography (TLC) or solid-phase extraction (SPE). The efficiency of a direct transesterification method was also evaluated. Additionally, the yield of the FAMEs and the profiles of the microalgae samples with different pretreatments (boiled in isopropanol, freezing, oven-dried and freeze-dried) were compared. The application of a purification step after lipid extraction proved to be essential for an accurate FAMEs characterisation. The purification methods, which included TLC and SPE, provided superior results compared to not purifying the samples. Freeze-dried microalgae produced the lowest FAMEs yield. However, FAMEs profiles were generally equivalent among the pretreatments.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available