Journal
BIORESOURCE TECHNOLOGY
Volume 253, Issue -, Pages 8-15Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.01.008
Keywords
Lipid production; Oleaginous yeast; Non-sterilized fermentation; Methanol; Crude glycerol
Funding
- Natural Sciences and Engineering Research Council of Canada (Canada Research Chair) [A 4984]
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In this work, methanol in crude glycerol solution was used to assist the lipid production with oleaginous yeast Trichosporon oleaginosus cultivated under non-sterilized conditions. The investigated methanol concentration was 0%, 1.4%, 2.2%, 3.3% and 4.4% (w/v). The results showed that methanol played a significant role in the non-sterilized fermentation for lipid production. The optimal methanol concentration was around 1.4% (w/v) in which the growth of T. oleaginosus was promoted and overcame that of the contaminants. The non-sterilized fed-batch fermentation with initial methanol concentration of 1.4% (w/v) was then performed and high biomass production (43.39 g/L) and lipid production (20.42 g/L) were achieved.
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