Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 41, Issue 7, Pages 1029-1038Publisher
SPRINGER
DOI: 10.1007/s00449-018-1932-1
Keywords
Aspergillus niger; Morphology; Citric acid; Chitin synthase; RNA interference
Funding
- National High-Tech Research and Development Program of China [2014AA021704]
- Natural Science Foundation of Anhui Province [1608085QC46]
- Major Projects of Science and Technology in Anhui Province [17030801036]
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The mycelial morphology of Aspergillus niger, a major filamentous fungus used for citric acid production, is important for citric acid synthesis during submerged fermentation. To investigate the involvement of the chitin synthase gene, chsC, in morphogenesis and citric acid production in A. niger, an RNAi system was constructed to silence chsC and the morphological mutants were screened after transformation. The compactness of the mycelial pellets was obviously reduced in the morphological mutants, with lower proportion of dispersed mycelia. These morphological changes have caused a decrease in viscosity and subsequent improvement in oxygen and mass transfer efficiency, which may be conducive for citric acid accumulation. All the transformants exhibited improvements in citric acid production; in particular, chsC-3 showed 42.6% higher production than the original strain in the shake flask. Moreover, the high-yield strain chsC-3 exhibited excellent citric acid production potential in the scale-up process.The citric acid yield and the conversion rate of glucose of chsC-3 were both improved by 3.6%, when compared with that of the original strain in the stirred tank bioreactor.
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