Alginate-Chitosan Micro- and Nanoparticles for Transmucosal Delivery of Proteins

The properties of protein-containing micro- and nanoparticles that were produced from alginate and chitosan using the methods of layer-by-layer polyelectrolyte adsorption and ionotropic gelation have been compared. The encapsulation efficiency of proteins (aprotinin, interferon, and human insulin), the size and zeta-potential of the particles, the mucin binding, and the protein release under physiological conditions have been studied. The prospects for the possible mucosal application of the particles are discussed.