Journal
BIOMATERIALS
Volume 167, Issue -, Pages 168-176Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2018.03.016
Keywords
Biofilms; Exopolysaccharides; Pseudomonas aeruginosa; Glycoside hydrolase; Biomaterials
Funding
- Canadian Institutes of Health Research [43998, 81361]
- Natural Sciences and Engineering Research Council of Canada [43594]
- Canada Foundation for Innovation [31799]
- Cystic Fibrosis Canada postdoctoral fellowship
- CIHR
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Bacterial colonization and biofilm formation on surfaces are typically mediated by the deposition of exopolysaccharides and conditioning protein layers. Pseudomonas aeruginosa is a nosocomial opportunistic pathogen that utilizes strain-specific exopolysaccharides such as Psl, Pel or alginate for both initial surface attachment and biofilm formation. To generate surfaces that resist P. aeruginosa colonization, we covalently bound a Psl-specific glycoside hydrolase (PslG(h)) to several, chemically-distinct surfaces using amine functionalization (APTMS) and glutaraldehyde (GDA) linking. In situ quartz crystal microbalance (QCM) experiments and fluorescence microscopy demonstrated a complete lack of Psl adsorption on the PslG(h)-bound surfaces. Covalently-bound PslG(h) was also found to significantly reduce P. aeruginosa surface attachment and biofilm formation over extended growth periods (8 days). The PslG(h) surfaces showed a similar to 99.9% (similar to 3-log) reduction in surface associated bacteria compared to control (untreated) surfaces, or those treated with inactive enzyme. This work demonstrates a non-eluting 'bioactive' surface that specifically targets a mechanism of cell adhesion, and that surface-bound glycoside hydrolase can significantly reduce surface colonization of bacteria through local, continuous enzymatic degradation of exopolysaccharide (Psl). These results have significant implications for the surface design of medical devices to keep bacteria in a planktonic state, and therefore susceptible to antibiotics and antimicrobials. (C) 2018 Elsevier Ltd. All rights reserved.
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