Journal
BIOMATERIALS
Volume 172, Issue -, Pages 14-29Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2018.01.054
Keywords
Fucoidan; VEGF; Polyelectrolyte multilayer film; Re-endothelialization; Antithrombotic; Heart valve tissue-engineering
Funding
- Direction de la Recherche et des Enseignements Doctoraux (Ministere de l'Enseignement Superieur et de la Recherche)
- University Paris 13
- Inserm (Institut National de la Sante et de la Recherche Medicate)
- Foundation for Cardiac Surgery Development
- University of Silesia
- Development of innovative bioactive prosthetic heart valve of the Polish National Centre for Research and Development [PBS3/A7/17/2015]
- Ministere de l'Enseignement Superieur et de la Recherche
- University Paris 13 (SREI)
- University Paris 13 (Bourse de Mobilite Internationale)
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Decellularized porcine heart valves offer promising potential as biocompatible prostheses. However, this procedure alter matrix fibres and glycans, leading to lower biomechanical resistance and increased their thrombotic potential. Therefore, their durability is limited due to calcification and weak regeneration in vivo. Surface modifications are highly requested to improve the scaffolds re-endothelialization required to restore functional and haemocompatible heart valve. Fucoidan, a natural sulphated polysaccharide, carries antithrombotic and anti-inflammatory properties and is known to enhance endothelial adhesion and proliferation when associated with vascular endothelial growth factor (VEGF). Based on these features, we constructed fucoidan/VEGF polyelectrolyte multilayer film (PEM) coated valve scaffold in an attempt to develop functional heart valve bioprosthesis. We investigated the haemo-compatibility of the PEM coated valve scaffolds, the adhesion and growth potential of endothelial cells (HUVECs) in flow, as well as long term culture with stem cells. Fucoidan/VEGF PEM coated scaffolds demonstrated antithrombotic and non-calcifying properties. The PEM application increased HUVECs adhesion in flow (6 h) and HUVECs viability over time (72 h). HUVECs were well spread and aligned in flow direction. Interestingly, stem cells infiltration was improved by the PEM coating at 21 days. Thus, the fucoidan/VEGF PEM is a promising surface modification to obtain valve bioprostheses for clinical applications with increased antithrombotic and re-endothelialization potential. (C) 2018 Published by Elsevier Ltd.
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