Journal
BIOMATERIALS
Volume 166, Issue -, Pages 96-108Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2018.03.006
Keywords
Fibronectin; Fibrillogenesis; Rotary jet spinning; Nanofiber; Wound healing; Hair follicle
Funding
- National Science Foundation under NSF [1541959]
- Harvard Medical School Department of Neurobiology Imaging Facility
- Neural Imaging Center, NINDS P30 Core Center grant [NS072030]
- NCI Cancer Center Support Grant [NIH 5 P30 CA06516]
- Harvard Materials Research Science and Engineering Center [DMR-1420570]
- NATIONAL CANCER INSTITUTE [P30CA006516] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [P30NS072030] Funding Source: NIH RePORTER
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Wounds in the fetus can heal without scarring. Consequently, biomaterials that attempt to recapitulate the biophysical and biochemical properties of fetal skin have emerged as promising pro-regenerative strategies. The extracellular matrix (ECM) protein fibronectin (Fn) in particular is believed to play a crucial role in directing this regenerative phenotype. Accordingly, Fn has been implicated in numerous wound healing studies, yet remains untested in its fibrillar conformation as found in fetal skin. Here, we show that high extensional (similar to 1.2 x 10(5) s(-1)) and shear (similar to 3 x 10(5) s(-1)) strain rates in rotary jet spinning (RJS) can drive high throughput Fn fibrillogenesis (similar to 10 mL/min), thus producing nanofiber scaffolds that are used to effectively enhance wound healing. When tested on a full-thickness wound mouse model, Fn nanofiber dressings not only accelerated wound closure, but also significantly improved tissue restoration, recovering dermal and epidermal structures as well as skin appendages and adipose tissue. Together, these results suggest that bioprotein nanofiber fabrication via RJS could set a new paradigm for enhancing wound healing and may thus find use in a variety of regenerative medicine applications. (C) 2018 Elsevier Ltd. All rights reserved.
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