Journal
BIOMATERIALS
Volume 167, Issue -, Pages 1-14Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2018.03.012
Keywords
Tissue engineering; Bio-3D printer; Scaffold-free cellular patch; Congenital diaphragmatic hernia; Transplantation; Skeletal muscle regeneration
Funding
- JSPS KAKENHI, Japan [25253094]
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Neonates with congenital diaphragmatic hernia often require surgical defect closure with a patch. Alternatives to native diaphragmatic tissue are critically needed for this paediatric surgery. The clinical efficacy of mesh patches is limited by complications associated with residual foreign material and by hernia recurrence. In this study, we used a novel bio-3D printer method to generate large scaffold-free tissue patches composed of human cells. The resulting large tissue constructs had high elasticity and strength. Cellular patches were transplanted into rats with surgically created diaphragmatic defects. Rats survived for over 710 days after implantation of tissue constructs. CT confirmed complete tissue integration of the grafts during rat growth. Histology revealed regeneration of muscle structure, neovascularization, and neuronal networks within the reconstructed diaphragms. Our results demonstrate that created cellular patches are a highly safe and effective therapeutic strategy for repairing diaphragmatic defects, and thus pave the way for a clinical trial. (C) 2018 The Authors. Published by Elsevier Ltd.
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