Journal
BIOLOGY OF REPRODUCTION
Volume 100, Issue 1, Pages 175-186Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/biolre/ioy162
Keywords
PGE(2) accumulation; COX-2; mPGES-1; EP4; bacterial infection; inflammatory damage
Categories
Funding
- National Natural Science Foundation of China [31672603]
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Endometritis is the most common bovine uterine disease following parturition. The role of prostaglandin E-2 (PGE(2)) in the regulation of endometrial inflammation and repair is well understood. Excess PGE(2) is also generated in multiple inflammatory diseases, including endometritis. However, it remains unclear whether PGE(2) is associated with pathogen-induced inflammatory damage to the endometrium. To clarify the role of PGE(2) in pathogen-induced inflammatory damage, this study evaluated the production of PGE(2), inflammatory factors, and damage-associated molecular patterns (DAMPs) in cultured Escherichia coli-infected bovine endometrial tissue. PGE(2) production was significantly higher in E. coli-infected tissue, and in E. coli-infected tissue treated with 15-prostaglandin dehydrogenase (15-PGDH) inhibitors, as compared to uninfected tissue. Phospholipase A2 (PLA2), cyclooxygenase-2 (COX-2), and microsomal prostaglandin E synthase-1 (mPGES-1) were also upregulated in E. coli-infected tissue, while concentrations of arachidonic acid (AA), leukotrienes, DAMPs, and other proinflammatory factors increased. The accumulation of PGE(2) clearly damaged the cultured tissue. Treatment with the COX-2, mPGES-1, EP4, and protein kinase A (PKA) inhibitors decreased the production of PGE(2), inflammatory factors, and DAMPs, simultaneously alleviating the E. coli-induced endometrial tissue damage. Therefore, the PGE(2) that was generated by COX-2 and mPGES-1 accumulated, and this pathogenic PGE(2) increased inflammatory damage by upregulating inflammatory factors and DAMPs in E. coli-infected bovine endometrial tissue. This upregulation of inflammatory factors and DAMPs might be regulated by the EP4-PKA signaling pathway. Escherichia coli infection of the bovine uterine explants activated the COX2-mPGES-PGE2 pathway, leading to the accumulation of PGE2, which induced proinflammatory cytokines IL-beta, IL-6, IL-8, TNF-alpha expression and tissue injure via PGE2-EP4-PKA pathway.
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