Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 41, Issue 5, Pages 733-742Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.b17-00919
Keywords
exosome secretion; stimulation; liposome; PEGylation; cationic lipid
Categories
Funding
- Egyptian Government
- research program for development of intelligent Tokushima artificial exosome (iTEX) from Tokushima University
- Takeda Science Foundation
- Uehara Memorial Foundation
- Takahashi Industrial and Economic Research Foundation
Ask authors/readers for more resources
Exosomes are tiny extracellular vesicles that are usually harvested in small quantities. Such small yield has been an obstacle for the expansion of the basic research regarding exosome analysis and applications in drug delivery. To increase exosome yield, we attempted to stimulate tumor cells via the addition of liposomes in vitro. Neutral, cationic-bare or PEGylated liposomes were incubated with four different tumor cell lines. The stimulatory effect of liposomal formulations on exosome secretion and cellular uptake propensity of the collected exosome by mother cells or different cells was evaluated. Both neutral and cationic-bare liposomes enhanced exosome secretion in a dose-dependent manner. Fluid cationic liposomes provided the strongest stimulation. Surprisingly, the PEGylation of bare liposomes diminished exosome secretion. Exosomes harvested in the presence of fluid cationic liposomes showed increased cellular uptake, but solid cationic liposomes did not. Our findings indicate that the physicochemical properties of liposomes determine whether they will act as a stimulant or as a depressant on exosome secretion from tumor cells. Liposomal stimulation may be a useful strategy to increase exosome yield, although further preparation to increase the purity of exosomes may be needed. In addition, fine-tuning of the biological properties of induced exosomes could be achieved via controlling the physicochemical properties of the stimulant liposomes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available