Journal
BIOELECTROCHEMISTRY
Volume 120, Issue -, Pages 150-156Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2017.12.009
Keywords
Electrochemical biosensor; Rose petal templates; Mesoporous TiO2; Horseradish peroxidase; H2O2 detection
Funding
- National Natural Science Foundation of China [21475115]
- Program for University Innovative Research Team of Henan [15IRTSTHN001]
- Henan Provincial Science and technology innovation team [C20150026]
- Nanhu Scholars Program of XYNU
- Nanhu Scholars Program for Young Scholars of XYNU
- Natural Science Foundation of Henan Province [162300410230]
- Henan Science and Technology Cooperation Project [172106000064]
- Research Starting Foundation for High Level Talent of XYNU
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In this work, highly ordered macro-mesoporous TiO2 has been successfully synthesized using fresh rose petals and P123 (EO20PO70EO20) as dual templates through a simple soaking and calcining process. Characterization of the as-prepared TiO2 indicated that the mesoporous structure of the TiO2 was highly ordered, with a pore diameter of approximately 3 nm. After electrodeposition of Pt nanoparticles onto the TiO2 as an electron transfer enhancer and the immobilization of horseradish peroxidase (HRP) onto the TiO2-modified electrode, a biosensor for detecting hydrogen peroxide (H2O2) was realized. This biosensor showed a wide linear detection range from 5 mu M to 8 mM and a low detection limit of 1.65 mu M with good stability and high selectivity, suggesting that the sensor is well-suited for the detection of H2O2. (C) 2017 Elsevier B.V. All rights reserved.
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