Journal
BIOCONJUGATE CHEMISTRY
Volume 29, Issue 8, Pages 2786-2792Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.8b00412
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Funding
- World Premier International Research Center Initiative (WPI), MEXT Japan
- JSPS KAKENHI [26119001, 26286028, 26220602, 18H01838]
- AMED Core Research for Evolutionary Medical Science and Technology (AMED CREST) [JP16gm0510004]
- Japan Science and Technology Agency under Core Research for Evolutional Science and Technology (JST CREST, Japan) [JPMJCR1333]
- Precursory Research for Embryonic Science and Technology (PRESTO) [JPMJPR14F1]
- Japan Society for Promotion of Science under the Funding Program for Next-Generation World Researchers (NEXT Program), Sasakawa Scientific Research Grant
- Grants-in-Aid for Scientific Research [26286028, 26119001, 18H01838] Funding Source: KAKEN
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Fluorescent nanodiamonds (FNDs) have been attracting much attention as promising therapeutic agents and probes for bioimaging and nanosensing. For their biological applications, several hydrophilizing methods to enhance FND colloidal stability have been developed to suppress their aggregation and the nonspecific adsorption to biomolecules in complex biomedical environments. However, these methods involve several complicated synthetic and purification steps, which prohibit the use of FNDs for bioapplications by biologists. In this study, we describe a simple one-pot FND hydrophilization method that comprises coating of the surface of the nanoparticles with COOH-terminated hyperbranched polyglycerol (HPG-COOH). HPG-COOH-coated FNDs (FND-HPG-COOHs) were found to exhibit excellent dispersibility under physiological conditions despite the thinness of the 5 nm HPG-COOH layer. Biotinylated FND-HPG-COOHs specifically captured avidin molecules in the absence of nonspecific protein adsorption. Moreover, we demonstrated that FND-HPG-COOHs conjugated with antibodies can be used to selectively target integrins in fixed HeLa cells. In addition, intracellular temperature changes were measured via optically detected magnetic resonance using FND-HPG-COOHs conjugated with mitochondrial localization signal peptides. Our one-pot synthetic method will encourage the broad use of FNDs among molecular and cellular biologists and pave the way for extensive biological and biomedical applications of FNDs.
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