Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1859, Issue 10, Pages 1067-1074Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2018.06.009
Keywords
Chloroplastic ATP synthase; Electron transfer; Leaf maturation; Photoprotection; Photosynthesis; Proton motive force
Categories
Funding
- National Natural Science Foundation of China [31670343]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2016347]
- Scientific and Technological Leading Talent Project of Yunnan Province [2016HA005]
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In the present study, we studied the role of chloroplastic ATP synthase in photosynthetic regulation during leaf maturation. We measured gas exchange, chlorophyll fluorescence, P700 redox state, and the electrochromic shift signal in mature and immature leaves. Under high light, the immature leaves displayed high levels of non-photochemical quenching (NPQ) and P700 oxidation ratio, and higher values for proton motive force (pmf) and proton gradient (Delta pH) across the thylakoid membranes but lower values for the activity of chloroplastic ATP synthase (g(H)(+)) than the mature leaves. Furthermore, g(H)(+) was significantly and positively correlated with CO2 assimilation rate and linear electron flow (LEF), but negatively correlated with pmf and Delta pH. Delta pH was significantly correlated with LEF and the P700 oxidation ratio. These results indicated that g(H)(+) was regulated to match photosynthetic capacity during leaf maturation, and the formation of pmf and Delta pH was predominantly regulated by the alterations in g(H)(+). In the immature leaves, the high steady-state Delta pH increased lumen acidification, which, in turn, stimulated photoprotection for the photosynthetic apparatus via NPQ induction and photosynthetic control. Our results highlighted the importance of chloroplastic ATP synthase in optimizing the trade-off between CO2 assimilation and photoprotection during leaf maturation.
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