Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 502, Issue 4, Pages 450-455Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2018.05.168
Keywords
tRNA; Codon usage; Efficiency of translation; Enterobacteria; Salmonella enterica; Escherichia coli
Categories
Funding
- Fondo Nacional de Desarrollo Cientifico y Tecnologico [11140222]
- Comision Nacional de Investigacion Cientifica y Tecnologica [79130044]
- Comision Nacional de Investigacion Cientifica y Tecnologica [Beca Doctorado Nacional scholarship] [21151441]
- National Institutes of Health [GM65183]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM065183] Funding Source: NIH RePORTER
Ask authors/readers for more resources
It is widely believed that if a high number of genes are found for any tRNA in a rapidly replicating bacteria, then the cytoplasmic levels of that tRNA will be high and an open reading frame containing a higher frequency of the complementary codon will be translated faster. This idea is based on correlations between the number of tRNA genes, tRNA concentration and the frequency of codon usage observed in a limited number of strains as well as from the fact that artificially changing the number of tRNA genes alters translation efficiency and consequently the amount of properly folded protein synthesized. tRNA gene number may greatly vary in a genome due to duplications, deletions and lateral transfer which in turn would alter the levels and functionality of many proteins. Such changes are potentially deleterious for fitness and as a result it is expected that changes in tRNA gene numbers should be accompanied by a modification of the frequency of codon usage. In contrast to this model, when comparing the number of tRNA genes and the frequency of codon usage of several Salmonella enterica and Escherichia coli strains we found that changes in the number of tRNA genes are not correlated to changes in codon usage. Furthermore, these changes are not correlated with a change in the efficiency of codon translation. These results suggest that once a genome gains or loses tRNA genes, it responds by modulating the concentrations of tRNAs rather than modifying its frequency of codon usage. (C) 2018 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available