Journal
FEBS LETTERS
Volume 589, Issue 9, Pages 974-984Publisher
WILEY
DOI: 10.1016/j.febslet.2015.02.035
Keywords
Human telomerase reverse transcriptase; Telomerase; Human telomerase RNA; Transcription; Telomerase components; Quantification
Funding
- core budget from Institute of Molecular and Cell Biology (IMCB)
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Besides its canonical function of catalyzing the formation of telomeric repeats, many groups have recently reported non-canonical functions of hTERT in particular, and telomerase in general. Regulating transcription is the central basis of non-canonical functions of telomerase. However, unlike reverse transcriptase activity of telomerase that requires only a few molecules of enzymatically active hTERT, non-canonical functions of hTERT or other telomerase components theoretically require several hundred copies. Here, we provide the first direct quantification of all the telomerase components in human cancer cell lines. We demonstrate that telomerase components do not exist in a 1:1 stoichiometric ratio, and there are several hundred copies of hTERT in cells. This provides the molecular basis of hTERT to function in other signaling cascades, including transcription. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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