Journal
FEBS LETTERS
Volume 589, Issue 17, Pages 2257-2265Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2015.06.038
Keywords
Insulin-like growth factor-2-mRNA-binding; protein (IGF2BP or IMP); miRNA or miR; miR-1275; Hepatocellular carcinoma; Posttranscriptional regulation; Insulin-like growth factor 1 receptor (IGF1R)
Funding
- Egyptian Science and Technology Development Fund (STDF) Grant [4242]
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This study aimed to identify a single miRNA or miR (microRNA) which regulates the three insulin-like growth factor-2-mRNA-binding proteins (IGF2BP1, 2 and 3). Bioinformatics predicted miR-1275 to simultaneously target the three IGF2BPs, and screening revealed miR-1275 to be underexpressed in hepatocellular carcinoma (HCC) tissues. Transfection of HuH-7 cells with miR-1275 suppressed IGF2BPs expression and all three IGF2BPs were confirmed as targets of miR-1275. Ectopic expression of miR-1275 and knockdown of IGF2BPs inhibited malignant cell behaviors, and also reduced IGF1R protein and mRNA. Finally IGF1R was validated as a direct target of miR-1275. These findings indicate that the tumor-suppressor miR-1275 can control HCC tumor growth partially through simultaneously regulating the oncogenic IGF2BPs and IGF1R. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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