Journal
FEBS LETTERS
Volume 589, Issue 19, Pages 2784-2790Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2015.08.022
Keywords
Symbiosis; AmiC; N-acetylmuramyl-L-alanine amidase; Riptortus pedestris; Burkholderia
Funding
- Pusan National University
- Grants-in-Aid for Scientific Research [26117732, 15K14586, 15H05638] Funding Source: KAKEN
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We generated a Burkholderia mutant, which is deficient of an N-acetylmuramyl-L-alanine amidase, AmiC, involved in peptidoglycan degradation. When non-motile Delta amiC mutant Burkholderia cells harboring chain form were orally administered to Riptortus insects, Delta amiC mutant cells were unable to establish symbiotic association. But, Delta amiC mutant complemented with amiC gene restored in vivo symbiotic association. Delta amiC mutant cultured in minimal medium restored their motility with single-celled morphology. When Delta amiC mutant cells harboring single-celled morphology were administered to the host insect, this mutant established normal symbiotic association, suggesting that bacterial motility is essential for the successful symbiosis between host insect and Burkhoideria symbiont. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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