Journal
FEBS LETTERS
Volume 589, Issue 21, Pages 3302-3308Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2015.09.024
Keywords
MicroRNA; Osteoblast; Core binding factor beta
Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Grants-in-Aid for Scientific Research [25462381] Funding Source: KAKEN
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Osteoblastic differentiation is regulated by various factors, including hormones and transcription factors. Runt-related transcription factor 2 (Runx2) is an essential player in osteoblastogenesis and transactivates its molecular target by creating a protein complex with its hetero-dimeric partner core binding factor beta (Cbfb). However, the molecular regulation of Cbfb expression remains unknown. Here, we identified miR-145 as a crucial regulator of Cbfb expression. The expression of miR-145 increased during osteoblastogenesis, indicating that miR-145 works as an inhibitor of osteoblastogenesis. Stable expression of miR-145 decreased endogenous Cbfb expression and inhibited osteoblastogenesis, in cooperation with miR-34c. Furthermore, miR-145 decreased bone regeneration in vivo. Our results indicate that miR-145 physiologically regulates osteoblast differentiation and bone formation via Cbfb expression by forming a regulatory microRNA network. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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