Journal
FEBS LETTERS
Volume 589, Issue 20, Pages 3133-3140Publisher
WILEY
DOI: 10.1016/j.febslet.2015.08.026
Keywords
Regulation; Cellulosome; Biomass sensing; Xylanase; Alternative sigma factor; Anti-sigma factor; Clostridium thermocellum
Funding
- US-Israel Binational Science Foundation (BSF) [2011049]
- Israel Science Foundation (ISF) [715/12, 500/10, 1349/13, 24/11]
- Sidney E. Frank Foundation through the ISF
- Ministry of Environmental Protection, Israel
- Israeli Center of Research Excellence (I-CORE Center) Program of the Planning and Budgeting Committee [152/11]
- Grand Technion Energy Program, The Leona M. and Harry B. Helmsley Charitable Trust
- BioEnergy Science Center (BESC)
- Office of Biological and Environmental Research in the DOE Office of Science
- Direct For Biological Sciences
- Div Of Biological Infrastructure [2011049] Funding Source: National Science Foundation
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Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the sigma(I6)/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative sigma(I6) and sigma(A) vegetative promoters. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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