Journal
FEBS LETTERS
Volume 589, Issue 18, Pages 2359-2366Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2015.07.035
Keywords
Glycosyltransferase; Regulation of gene expression; Fucosyltransferase; Hypoxia inducible factor 1 alpha (HIF-1 alpha); Cancer
Funding
- Portuguese Foundation for Science and Technology [SFRH/BD/44820/2008]
- Netherlands Institute for Regenerative Medicine
- Dutch Cancer Society [VU2014-6779]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/44820/2008] Funding Source: FCT
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The alpha 1,2-fucosyltransferase activity in pancreatic tumors is much lower compared to normal pancreatic tissue. Here we show that hypoxia inducible factor (HIF) 1 alpha is constitutively expressed in the pancreatic cancer cell lines Pa-Tu-8988S and Pa-Tu-8988T and suppresses the expression of the alpha 1,2-fucosyltransferase genes FUT1 and FUT2. Down regulation of HIF-1 alpha expression resulted in elevated FUT1 and FUT2 transcript levels and an increased expression of alpha 1,2-fucosylated glycan structures on the surface of these cells. In conclusion, our data are the first to identify HIF-1 alpha as a suppressor of FUT1/2 expression, thereby regulating alpha 1,2-fucosylation of cell-surface glycans. (C) 2015 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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