Journal
AVIAN PATHOLOGY
Volume 47, Issue 3, Pages 245-252Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/03079457.2017.1416590
Keywords
TaqMan fluorescent quantitative PCR; probe; detection; poultry; prevalence
Categories
Funding
- Key Discipline of Preventive Veterinary Medicine of Henan University of Animal Husbandry and Economy of China [MXK2016102]
- Technology Innovation Team Project from Henan University of Animal Husbandry and Economy of China [HUAHE2015014]
- National Natural Science Foundation of China [U1404328]
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To better understand the prevalence of Gallibacterium anatis in different poultry species, a rapid and accurate method was developed to detect G. anatis using a TaqMan fluorescent quantitative polymerase chain reaction (qPCR). Specific primers and a TaqMan probe were designed based on the reference gtxA gene sequence. The qPCR standard curve showed a good linear relationship, and the method showed good reproducibility, sensitivity, and specificity, indicating its suitability for G. anatis identification and quantitative analysis. A comparison of the detection results in 160 clinical swab samples showed that the detection rate (54.4%) of the qPCR for G. anatis was better than that of two conventional methods: gyrB gene-based qPCR for G. anatis (51.9%) and culture-based identification (34.4%). G. anatis was detected in layer chicken (77.3%), Silkie chicken (72.7%), and duck (27.1%) with relatively high detection rates, whereas dove (8.8%) and quail (3.0%) showed lower detection rates, indicating the different prevalence of G. anatis in different fowl species.
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